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When should you take BC?

Cryo-sections (7 μm) were cut from the left lung, dried onto glass slides, and stored at −20 °C. Frozen sections were post-fixed with 3.7% phosphate-buffered formaldehyde for 10 min. Sections were rinsed in phosphate-buffered saline (PBS), followed by quenching of endogenous peroxidase with 1% H₂O₂ in PBS for 10 min and treated with 3% bovine serum albumin (fraction V) for 1 h at room temperature. An anti-CD68 antibody (AbDSerotec, diluted 1:100 in PBS, 1% BSA) and an anti-pro-surfactant protein C antibody (antibodies-online, diluted 1:400 in PBS, 1% BSA) was used to label alveolar macrophages and type-2 epithelial cells, respectively. Rabbit anti-nitrotyrosin antibody (Merck Millipore No.: 06284, diluted 1:200) was used to label nitrosylated proteins. Bound specific antibodies were labelled with biotinylated anti-mouse IgG (Jackson Immuno Research Labs, West Grove, PA, USA) to detect CD68, and biotinylated anti-rabbit IgG (Vector Laboratories, Burlingame, CA, USA) to detect surfactant protein C and nitrotyrosine. Avidin-biotin horse radish peroxidase-based detection systems (Vector Laboratories, No.: PK-4001) followed by substrate staining (Vector blue for CD68 and nitrotyrosine, Vector laboratories, No.: SK-4700) and AEC for surfactant protein C (Vector Laboratories, No.: SK-4205) were used. Nitrosylated tissue was prepared as a positive control by bathing sections in a mixture of 1 mM NaNO₂/1 mM H₂O₂ in 100 mM sodium acetate, pH 5. All sections were finally rinsed in phosphate buffered saline (PBS) and cover slipped with aqueous mounting medium (Immu-Mount, Fisher Scientific GmbH, Schwerte, Germany). Micrographs were taken with a Retiga 2000R camera and Q capture imaging software (Q Imaging Corporation, Surrey, BC, Canada) mounted on an Olympus BX51 microscope (Olympus Germany GmbH, Hamburg, Germany). Unstained and immuno-stained sections were also viewed with enhanced dark field microscopy (DFM) using an appropriate DFM condenser (CytoViva Inc., Auburn, AL, USA). Polarization microscopy was carried out with two perpendicularly oriented linear polarizing filters, which were inserted into the optical path of the microscope.

The method of capturing CO₂ after the combustion process consists in separating carbon dioxide from other substances in the exhaust gas, such as nitrogen oxides, nitrogen or sulfur oxides. There are many methods of CO